Investigating Enzymes

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... gen produced. The results will then be recorded in a table, and then graphed after the experiment has been conducted at a satisfactory amount of temperatures. My preliminary experiment suggests that suitable quantities to use would be 20cm3 hydrogen peroxide and 10cm3 leek, timed over a period of one minute during the reaction. I am now able to perform the experiment at a range of temperatures between 0degrees and 80degrees. If the rate of reaction is still above zero beyond 80degrees, I will continue the experiment for higher temperatures. My research suggests however that most enzymes become denatured before 80degrees.

Fair Testing

To make sure this experiment is a fair test, the only factor I must vary must be temperature. This means that I have to keep the concentrations, pressure and pH of the substance constant throughout the experiment. In order to ensure accuracy, I will conduct the experiment three times for each temperature and take a mean result to plot on the graph. The exact quantities used must first be determined by a preliminary experiment.

Results

Conclusion

Several points can be drawn from the results of this experiment. The first is that the graph is very similar to that of my prediction. It clearly shows an optimum temperature at which th ...

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