Investigating the rate of reaction of enzymes in the clearing of photographic film
To read all this essay and more Visit Coursework.info
Below is a short sample of the document. Visit Coursework.info for the full version.
I will also need to make sure that the photographic film is completely clear before I stop the timer. When I think it is clear I will press the red button on the timer and if the film goes no clearer I will take the time that I paused the timer at. If it does go clearer I will continue the timer and stop it when it goes clearer. Safe Test
As well as the experiment needing to be a fair test, it must also be a safe test for it to be successful. I must make sure that safety goggles are worn at all times in the experiment as trypsin would be very damaging if it got into the eye as it breaks down protein and most of the eye is made of protein. Also as a bunsen burner is to be used in the experiment I must be careful when it is alight. Prediction
I will now predict what will happen at the different pH's that I am going to study (pH 5, 6, 7, 8, 9 and 10).
The rate of reaction should be slower when the pH is lower than 7 as there are more enzymes becoming denatured at lower pHs. There should be a faster rate of reaction as the pH goes up until it is at 7 where the enzyme works best. When the pH goes higher than 7 the rate of reaction will be slower as more enzymes start to become denatured.
From this, I can predict that when the resu ...
